Generator

Part:BBa_K638301:Design

Designed by: Matthew Jones   Group: iGEM11_Cambridge   (2011-09-19)


Arabinose inducible Reflectin A1-sfGFP fusion generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2307


Design Notes

We deliberately arranged the sfGFP part of the fusion on the C terminus of the protein as translation is known to proceed from N terminus to C terminus. This means that any sfGFP visualised in vivo must have been produced with reflectin A1 synthesised first, then the sfGFP component.


Source

This composite part was built using gibson assembly to add Reflectin A1 between pBAD and sfGFP on an existing pBAD-sfGFP construct. BBa_I0500 - Registry Part BBa_B0034 - Registry Part BBa_K638001 - Codon optimised Reflectin A1 Supplied by Wendy Crookes BBa_I746916 - Registry Part BBa_B0010 - Registry Part BBa_B0012 - Registry Part

References